ABSTRACT
In order to enrich the transcriptome data of Fagopyrum dibotrys plants, analyze the genes encoding key enzyme involved in flavonoid biosynthesis pathway, and mine their functional genes, in this study, we performed RNA sequencing analysis for the rhizomes, roots, flowers, leaves and stems of F. dibotrys on the BGISEQ-500 sequencing platform. After de novo assembly of transcripts, a total of 205 619 unigenes were generated and 132 372 unigenes were obtained and annotated into seven public databases, of which, 81 327 unigenes were mapped to the GO database and most of the unigenes were annotated in cellular process, biological regulation, binding and catalytic activity. Besides, 86 922 unigenes were enriched in 136 pathways using KEGG database' and we identified 82 unigenes that encodes key enzymes involved in flavonoid biosynthesis. Comparing rhizome with root, flower, leaf or stem in F. dibotrys, 27 962 co-expressed differentially expressed genes(DEGs) were obtained. Among them, 23 515 DEGs of rhizome tissue-specific were enriched into 132 pathways and 13 unigenes were significantly enriched in biosynthesis of flavone and flavonol. In addition, we also identified 3 427 unigenes encoding 60 transcription factor(TFs) families as well as four unigenes encoding bHLH TFs were enriched in flavonoid biosynthesis. Our results greatly enriched the transcriptome database of plants, provided a reference for the analysis of key enzymes involved in flavonoid biosynthesis in plants, and will facilitate the study of the functions and regulatory mechanisms of key enzymes involved in flavonoid biosynthesis in F. dibotrys at the genetic level.
Subject(s)
Humans , Biosynthetic Pathways/genetics , Fagopyrum , Flavonoids , Flowers , Gene Expression Profiling , Gene Expression Regulation, Plant , Transcriptome/geneticsABSTRACT
OBJECTIVE：To study the pharmacological effects and possible molecular mechanism of the superior components in Fagopyrum dibotrys . METHODS ：Based on network pharmacology ，by selecting DL ＞0.18 and OB ＞30％ as criteria ，superior components of F. dibotrys were screened out ，using the traditional Chinese medicine integrated pharmacology （TCMIP）platform. Pharm Mapper database was utilized to obtain the potential targets of each components ；Kyoto gene and genome database （KEGG） signal pathway analysis and gene ontology （GO）bioprocess enrichment analysis were performed for target protein with DAVID database（all using P＜0.05 as criteria ）. RESULTS ：15 kinds of superior components [such as quercetin ，luteolin，procyanidin B 1， （+）catechin and β-sitosterol，etc.] and 114 target proteins （such as estradiol 17-β-dehydrogenase 1，cAMP specific 3，5-cyclic phosphodiesterase 4D，vitamin D 3 receptor，uridine cytidine kinase 2，urokinase triphosphate encoded by HRAS gene ，etc.）were screened out ，involving 34 important pathways ，like MAPK signaling pathway ，VEGF signaling pathway ，chemokine signaling pathway and insulin signaling pathway ；among them ，11 were cancer-related pathways ，7 were metabolic-related pathways ，4 were endocrine-related pathways. The involved molecular functions included steroid receptor activity ，ligand dependent nuclear receptor activity，protein kinase activity ，etc.；cell components included cell fluid ，cell fragment ，soluble part ，etc.；biological processes included the regulation of apoptosis ，the process of organic response ，the process of endogenous stimulation response ，etc. CONCLUSIONS：F. dibotrys may play anti-tumor effects ，anti-inflammatory effects ，regulation of glycolipid metabolism by acting on MAPK signaling pathway ，VEGF signaling pathway ，chemokine signaling pathway and insulin signaling pathway.
ABSTRACT
Objective: To study the chemical constituents from the root tubers of Fagopyrum dibotrys. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fourteen known compounds were isolated from methanol extract in the dry roots of F. dibotrys and thier structures were identified as luteolin (1), tricin (2), luteolin-7,4'-dimethyl ether (3), quercetin (4), genkwanin (5), chrysoeriol (6), protocatechuic acid (7), protocatechuic acid methyl ester (8), p-hydroxybenzaldehyde (9), glutinone (10), glutinol (11), olean-12-ene-3β, 7β, 15α, 28-tetraol (12), juglangenin A (13), and 21β-dihydroxy-olean-12-ene (14). Conclusion: Compounds 5 and 6 are firstly obtained from F. dibotrys. Compounds 12-14 are isolated from the plants of Fagopyrum Mill. for the first time.
ABSTRACT
Objective: To study the chemical constituents from the aerial parts of Fagopyrum dibotrys. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fourteen known compounds were isolated in the methanol extract from the aerial parts of F. dibotrys and their structures were identified as benzoic acid (1), p-hydroxybenzoic acid (2), p-hydroxy benzaldehyde (3), 3,4-dihydroxy benzoic acid (4), succinic acid (5), caffeic acid (6), methyl caffeate acid (7), luteolin (8), tricin (9), quercetin (10), afzelin A (11), 2α,3β,29-trihydroxyolean-12-en-28-oic acid (12), yarumic acid (13), and 3α-hydroxy-urs-12,15-dien (14). Conclusion: Compounds 2-3 and 6-9 are firstly obtained from the aerial parts of F. dibotrys. Compounds 11-14 are isolated from the genus of Fagopyrum Mill. for the first time.
ABSTRACT
Objective: To obtain the full-length cDNA sequence of anthocyanins synthase (ANS) gene from Fagopyrum dibotrys (FdANS), and to analyze the expression of FdANS in each tissue and the total anthocyanin content during florescence of F. dibotrys. Methods: The cDNA sequence of ANS gene was cloned by homology cloning from F. dibotrys. The expression of ANS was analyzed by semi-quantitative RT-PCR. The content of total anthocyanin was measured by spectrophotometry. Results: The open reading frame (ORF) of FdANS was 1077 bp and encoded a protein with 358 amino acids named FdANS. Bioinforamtion analysis suggested that the amino acid sequence of FdANS had the higher homology with those in other plants. Both the gene expression FdANS in different tissues during florescence of F. dibotrys and the total anthocyanin content were in the order of flowers > leaves > stems> roots. Conclusion: The cDNA sequence of FdANS is firstly obtained from F. dibotrys and its coding protein has the typical characteristic of ANS homologous protein. The gene expression of FdANS shows the correlation to the total anthocyanins content in the flowers, leaves, stems, and roots of F. dibotrys.
ABSTRACT
Objective: To clone and analyze the full-length cDNA of chalcone isomerase (CHI) gene from Fagopyrum dibotrys (FdCHI). To analyze the expression of CHI and the total flavonoids content during florescence of F. dibotrys. Methods: The cDNA sequence of CHI was cloned by homology cloning from F. dibotrys. The expression of CHI was analyzed in the different tissues during florescence by semi-quantitative RT-PCR. The content of total flavonoids was measured by AlCl3 method. Results: The open reading frame (ORF) of FdCHI was 750 bp and encoded a protein with 249 amino acids. Bioinforamtion analysis suggested that the amino acid sequence of FdCHI had the high homology with those in other plants. Gene expression analysis showed that FdCHI was highly expressed in the flowers, followed by the roots and leaves, while lower in the stems. The content of total flavonoids was the highest in the flowers then the leaves and stems, and the lowest was in the roots. Conclusion: The cDNA sequence of FdCHI is firstly obtained from F. dibotrys and its coding protein has the typical characteristic of CHI homologous protein. The gene expression of FdCHI shows the same to the total flavonoids content in the stems, leaves, and flowers, but different in the roots of F. dibotrys.